The effect of eight weeks of high-intensity interval training and curcumin supplementation on salivary 8-hydroxydeoxyguanosine and glutathione peroxidase levels in overweight women

Background and aims: Oxidative stress, a significant contributor to numerous diseases, including obesity, is explicitly targeted by high-intensity interval training (HIIT) and curcumin supplementation due to its antioxidant properties. This study aimed to investigate the effects of eight weeks of HIIT and curcumin supplementation on the salivary levels of 8-hydroxydeoxyguanosine (8-OHdG) and glutathione peroxidase (GPx) in overweight women. Methods: Forty-eight overweight women were randomly assigned to four groups: HIIT, HIIT+curcumin, curcumin, and placebo. The HIIT groups performed three sessions of HIIT per week for eight weeks, while the curcumin groups received 80 mg of curcumin per day for eight weeks. Salivary 8-OHdG and GPx levels were measured at baseline and after eight weeks. One-way analysis of variance with repeated measures was used to assess both interactive and between-group changes. Results: The results indicated that the interaction effect of the time×group was statistically significant for salivary 8-OHdG (P=0.001) but not for the salivary GPx (P=0.054). Salivary 8-OHdG and GPx levels showed significant decreases and increases, respectively, in the supplement (P=0.001), exercise+supplement (P=0.017), and exercise+placebo (P=0.001) groups. Conclusion: HIIT, along with curcumin supplementation, can reduce oxidative stress levels and effectively reduce inflammatory status and weight loss in overweight women.