Performance Evaluation of Five Real‐Time <scp>PCR</scp> Assays for the Detection of <i>Candida auris</i><scp>DNA</scp>
Real-time PCR is a crucial tool for the rapid and sensitive detection of C. auris , especially in clinical settings where timely identification is essential for effective patient management and infection control. Numerous PCR assays are available for this purpose; however, our study demonstrates that the sensitivity of these assays can vary significantly. The observed differences underscore the importance of establishing international reference standards and proficiency panels to enhance the accuracy and comparability of assay performance across different studies and laboratories.
Authors
- Willem J. G. Melchers (ORCID: https://orcid.org/0000-0002-5446-2230)
- Bart Van den Bosch (ORCID: https://orcid.org/0000-0002-0876-3227)
- Theun de Groot (ORCID: https://orcid.org/0000-0001-5841-6461)
- Eelco F. J. Meijer (ORCID: https://orcid.org/0000-0002-0226-024X)
- Jochem B. Buil (ORCID: https://orcid.org/0000-0003-4031-0778)
- Suzan D. Pas (ORCID: https://orcid.org/0000-0003-1943-1217)
- Stefanie Maas
- Joseph Meletiadis (ORCID: https://orcid.org/0000-0002-8869-1509)
- Paul E. Verweij (ORCID: https://orcid.org/0000-0002-8600-9860)
Institutions
- Statens Serum Institut (DK)
- Radboud University Nijmegen (NL)
- National and Kapodistrian University of Athens (GR)
- Erasmus MC (NL)
- Radboud University Medical Center (NL)
- National Institute for Public Health and the Environment (NL)
- Canisius-Wilhelmina Ziekenhuis (NL)
Publication Details
- Journal
- Mycoses
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1111/myc.70065
- Citations
- 2
- Primary Topic
- Antifungal resistance and susceptibility
- Type
- article
- Field-Weighted Citation Impact
- 2.79