A toolkit for facilitating markerless integration of expression cassettes in Komagataella phaffii via CRISPR/Cas9
The adapted GoldenPiCS toolkit combined with CRISPR/Cas9 technology enabled efficient and precise genome integration in K. phaffii. This approach holds promise for expanding the production of high-value recombinant proteins. Future research should focus on optimizing integration sites and improving cloning procedures to enhance the system's efficiency and versatility.
Authors
- Sissel Beate Rønning (ORCID: https://orcid.org/0000-0002-2255-0172)
- Laura García-Calvo (ORCID: https://orcid.org/0000-0002-9885-9854)
- Annette Fagerlund (ORCID: https://orcid.org/0000-0001-7146-4265)
- Charlotte Kummen
- Solvor Rustad
Institutions
- Nofima (NO)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-05-03
- DOI
- https://doi.org/10.1186/s12934-025-02716-x
- Citations
- 2
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 1.61
Funders
- Norges Forskningsråd