Use of polyethylene glycol precipitation and ultracentrifugation to enhance the sensitivity of hepatitis B virus DNA detection
PEG precipitation from large volumes of plasma is a practical and economical alternative to ultracentrifugation and could be a similarly effective concentration method for low viral load samples in blood donation and clinical virology laboratories.
Authors
- Richard Mayne (ORCID: https://orcid.org/0000-0003-1915-4993)
- Monique Andersson (ORCID: https://orcid.org/0000-0003-0619-1074)
- Osmany Larralde (ORCID: https://orcid.org/0000-0001-6838-5494)
- Lisa Jarvis
- Jane A. McKeating (ORCID: https://orcid.org/0000-0002-7229-5886)
- Heli Harvala (ORCID: https://orcid.org/0000-0001-9154-4190)
- Tanya Golubchik (ORCID: https://orcid.org/0000-0003-2765-9828)
- William L. Irving (ORCID: https://orcid.org/0000-0002-7268-3168)
- Michael X. Fu (ORCID: https://orcid.org/0000-0002-3108-7917)
- Peter Simmonds
- Kai Kean
- Kaitlin Reid
Institutions
- Nottingham University Hospitals NHS Trust (GB)
- The George Institute for Global Health (GB)
- Turku University Hospital (FI)
- University of Oxford (GB)
- Nottingham Biomedical Research Centre (GB)
- Oxford University Hospitals NHS Trust (GB)
- Scottish National Blood Transfusion Service (GB)
Publication Details
- Journal
- Journal of Clinical Virology
- Published
- 2025-05-03
- DOI
- https://doi.org/10.1016/j.jcv.2025.105802
- Citations
- 2
- Primary Topic
- SARS-CoV-2 detection and testing
- Type
- article
- Field-Weighted Citation Impact
- 2.79
Funders
- National Institute for Health and Care Research