MULTIPLE MYCOTOXIN ANALYSIS IN ARECA SEED OIL USING ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY (UPLC-MS/MS)

Analysis of mycotoxins in Areca seed oil faces is constrained by matrix complexities, such as fats and polar compounds, which affect extraction efficiency and accuracy.This research was performed to propose a method for extraction and analysis of mycotoxins in Areca seed oil using UPLC-MS/MS.Samples were three varieties of Areca cathecu seeds with solid phase extraction (SPE) using Carbon/Primary Secondary Amine (carb/PSA), followed by analysis using UPLC-MS/MS.The proposed method went through accuracy validation.The results of UPLC-MS/MS analysis with Carb/PSA extraction were proven optimal for detecting 24 mycotoxins with a high recovery rate (95-102%).The validation results fulfilled the requirements with a test range of 0.5-250 ng/g and a LOQ of 0.5 ng/g.Linearity R 2 >0.99 and % RSD <RSDmax for intra-and inter-day precision.Accuracy is assessed at LOQ (-50% to 20%), 10LOQ (-30% to 10%), and 100LOQ (-20% to 10%).There was no significant difference (p<0.05 for method precision with LC-ID-MS/MS and certified reference materials (CRM).AFB1 dominates in the three varieties of Areca catechu in Indonesia with a range of 2.56 -3.24 ng/g and other potential dangers from mycotoxins<LOQ such as ZEN, DON, T2, HT2, and OTA