Rapid identification and differentiation of blaGES cephalosporinase and carbapenemase types using real-time PCR assay
We have developed a rapid, sensitive and easily applicable method to detect and distinguish glycine, serine and asparagine bla<sub>GES</sub> variants using real-time PCR assays. These assays are suitable for routine use on cultured isolates but could, after further validation, even be used directly on screening broths. The assays will facilitate the detection and characterization of bla<sub>GES</sub> for both diagnostic purposes, antibiotic stewardship, infection prevention and control, and research purposes.
Authors
- Yulia R. Saharman
- Juliëtte A. Severin (ORCID: https://orcid.org/0000-0002-5222-3017)
- Corné H. W. Klaassen (ORCID: https://orcid.org/0000-0002-3439-0903)
- Lisa M. Meekes (ORCID: https://orcid.org/0009-0002-6140-2575)
Institutions
- Erasmus MC (NL)
- University of Indonesia (ID)
- Rumah Sakit Umum Pusat Nasional Dr. Cipto Mangunkusumo (ID)
- Erasmus University Rotterdam (NL)
Publication Details
- Journal
- Journal of Global Antimicrobial Resistance
- Published
- 2025-05-03
- DOI
- https://doi.org/10.1016/j.jgar.2025.04.029
- Citations
- 1
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.59