Standardization of DNA Extraction Protocol and Assessment of Molecular Genetic Variation in Honey Bees

This study aimed to standardize a DNA extraction protocol for honey bees, assess molecular diversity using microsatellite markers, and evaluate morphological diversity in honey bees. The modified DNA extraction protocol was successful in isolating high-quality genomic DNA from different body parts of Apis species. This DNA was suitable for PCR-based applications, including microsatellite marker-based genetic analysis. Morphological examination of the honey bee species revealed distinct variations, enabling the identification of the three Apis species based on measured characters. We present a simplified and efficient DNA extraction protocol for honey bees, which is recommended for genomic and microsatellite analysis. Additionally, morphological diversity analysis provides a reliable method for species identification, contributing to the understanding of honey bee diversity.