Highly Stable and Integrable Graphene/Molybdenum Disulfide Heterojunction Field-Effect Transistor-Based miRNA Biosensor
MicroRNAs (miRNAs) are important noncoding RNA molecules that participate in gene regulation and are widely associated with the occurrence and development of various cancers. Developing rapid, highly sensitive, low-cost, and highly stable miRNA detection methods is of great significance for clinical diagnosis. Field-effect transistors (FETs) based on two-dimensional (2D) materials have been proven to have great potential in the field of miRNA detection due to their label-free, rapid, highly sensitive, low-power, and portable features. However, biosensors based on 2D material FETs require the application of an external gate voltage in solution, which seriously hinders the integration, miniaturization, and signal stability of the devices. This study proposes a graphene-molybdenum disulfide heterojunction (G/MoS<sub>2</sub>) FET biosensing platform to detect miRNA-21 and miRNA-155 without the need for an external gate voltage. The results demonstrate a detection time of approximately 30 min, a linear response range spanning from 10 fM to 10 nM, and limits of detection of 6.06 fM for miRNA-21 and 2.59 fM for miRNA-155. Through comparative experiments, the biosensor shows excellent selectivity and can distinguish target miRNAs from nontarget miRNAs. The G/MoS<sub>2</sub> FET biosensor developed in this study provides a technical platform for miRNA detection and has a broad application prospect, especially in the early diagnosis of diseases and the screening of biomarkers.
Authors
- Xiangqian Hong (ORCID: https://orcid.org/0000-0002-4691-6669)
- Xilin Tian
- Ming‐Min Yang (ORCID: https://orcid.org/0000-0002-6628-5112)
- Yihan Zhu (ORCID: https://orcid.org/0000-0002-8150-7350)
- Lei Zhou (ORCID: https://orcid.org/0000-0002-2645-7901)
- Ming Gao (ORCID: https://orcid.org/0000-0003-3628-882X)
- Changle Meng
- Shuo Sun (ORCID: https://orcid.org/0000-0002-7178-0564)
- Yule Zhang
- Hao Huang (ORCID: https://orcid.org/0000-0001-9785-996X)
- Huide Wang (ORCID: https://orcid.org/0000-0003-0832-4774)
- Ziqian Wang (ORCID: https://orcid.org/0000-0002-0282-5941)
- Han Zhang (ORCID: https://orcid.org/0000-0002-0166-1973)
- Zhi Chen (ORCID: https://orcid.org/0000-0002-7624-3143)
- Jianqing Li (ORCID: https://orcid.org/0000-0002-6768-1483)
- Honghai Zhu
- Ke Jiang (ORCID: https://orcid.org/0000-0002-8372-1473)
- Ning Fan (ORCID: https://orcid.org/0000-0003-1673-911X)
- Yi Liu (ORCID: https://orcid.org/0009-0009-8693-0359)
- Yanqi Ge (ORCID: https://orcid.org/0000-0002-2055-1948)
- Yujie Zhou (ORCID: https://orcid.org/0000-0002-4528-6087)
- Meng Qiu (ORCID: https://orcid.org/0000-0002-3312-4873)
- Yun Wang (ORCID: https://orcid.org/0009-0006-5841-3627)
- Chen Wang (ORCID: https://orcid.org/0009-0004-4302-2565)
Institutions
- Macau University of Science and Technology (MO)
- Hangzhou Normal University (CN)
- Shenzhen University (CN)
- Chinese Academy of Sciences (CN)
- Changchun Institute of Optics, Fine Mechanics and Physics (CN)
- Southern Medical University Shenzhen Hospital (CN)
- Ocean University of China (CN)
Publication Details
- Journal
- ACS Applied Materials & Interfaces
- Published
- 2025-05-02
- DOI
- https://doi.org/10.1021/acsami.5c03827
- Citations
- 7
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 5.64
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Shandong Province
- Hangzhou Normal University
- Shenzhen University
- Taishan Scholar Foundation of Shandong Province
- Department of Education of Guangdong Province
- Science, Technology and Innovation Commission of Shenzhen Municipality
- State Key Laboratory of Luminescence and Applications
- China National Nuclear Corporation
- Basic and Applied Basic Research Foundation of Guangdong Province