Validation and Optimization of PURE Ribosome Display for Screening Synthetic Nanobody Libraries
Implementing a target protein elution step prior to EDTA-mediated disassembly and modifying the immobilization surfaces effectively increase selection efficiency. For PURE ribosome display, efficiency was further improved using a suitable spacer gene, enabling the display of large libraries.
Authors
- Daiwen Yang
- Bing‐Ying Liu (ORCID: https://orcid.org/0009-0003-3971-5206)
Institutions
- National University of Singapore (SG)
- Université Bourgogne Franche-Comté (FR)
Publication Details
- Journal
- Antibodies
- Published
- 2025-05-02
- DOI
- https://doi.org/10.3390/antib14020039
- Citations
- 2
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 2.72