From the archives: Nitrogen matters in crop domestication, rice nitrogen nutrition, and symbiotic nitrogen fixation

et al. ( 2020) recently uncovered a tripartite interaction among rice plants, the basidiomycetous yeast, 1 Rhodotorula mucilaginosa JGTA-S1, and its endophytic bacterial counterpart Pseudomonas stutzeri, 2 that improves plant N2 fixation.Although R. mucilaginosa has an incomplete N metabolic pathway, the 3 authors discovered that it can grow on N-free medium, which prompted them to investigate the presence 4 of endogenous N2-fixing bacteria, such as P. stutzeri.Amplification and sequencing analysis of the nifH