Multi-Layered Security Framework Combining Steganography and DNA Coding

With the rapid expansion of digital communication and data sharing, ensuring robust security for sensitive information has become increasingly critical, particularly when data are transmitted over public networks. Traditional encryption techniques are increasingly vulnerable to evolving cyber threats, making single-layer security mechanisms less effective. This study proposes a multi-layered security approach that integrates cryptographic and steganographic techniques to enhance data protection. The framework leverages advanced methods such as encrypted data embedding in images, DNA sequence coding, QR codes, and least significant bit (LSB) steganography. To evaluate its effectiveness, experiments were conducted using text messages, text files, and images, with security assessments based on PSNR, MSE, SNR, and encryption–decryption times for text data. Image security was analyzed through visual inspection, correlation, entropy, standard deviation, key space analysis, randomness, and differential analysis. The proposed method demonstrated strong resilience against differential cryptanalysis, achieving high NPCR values (99.5784%, 99.4292%, and 99.5784%) and UACI values (33.5873%, 33.5149%, and 33.3745%), indicating robust diffusion and confusion properties. These results highlight the reliability and effectiveness of the proposed framework in safeguarding data integrity and confidentiality, providing a promising direction for future cryptographic research.