A COMPARATIVE EVALUATION OF NORMAL SALINE, CHLORHEXIDINE, AND EDTA ON THE SEALING ABILITY OF BIODENTINE ON SIMULATED IMMATURE APEX- AN IN VITRO STUDY

Background: The study aims to evaluate the usefulness of biodentine as a root end-filling material following the removal of smear layers, as well as the impact of biodentine’s sealing ability when irrigated with normal saline, chlorhexidine, and EDTA. Objective: To evaluate and compare the sealing ability of biodentine after using various irrigating solutions on simulated immature apex. Methods: thirty extracted human maxillary central incisors were collected for the study with 10 samples per group. The apices were removed by cutting with a diamond disc- 2mm from the apical root end and the teeth were decoronated in an attempt to standardize the working length of all specimens to 15 mm. A standardized open apex was created using Peeso drills #1 to #6 in a way that drill #6 was allowed to pass 1 mm beyond the apex. All samples were randomly divided into 4 groups: Group–I: normal saline; Group–II: 2%chorhexidine; GroupIII:17%EDTA. After irrigation, 3 mm of apical plug was formed using biodentine in all the samples. Specimens were then immersed in 2% methylene blue for 48 hours after obturation with gutta-percha. The specimens were analyzed under a stereomicroscope at 20x magnification. The dye penetration was calculated using computer software. Result: The data was statistically analyzed using One Way ANOVA and Tukey’s post hoc test. The mean of dye penetration varied across the irrigants, with highest mean value obtained in Normal saline (2.71) followed by CHX (2.24), and EDTA (1.97). Conclusion: It was concluded that irrigation with EDTA significantly influenced the sealing ability of Biodentine followed by chlorhexidine and normal saline