Bioinformatic challenges in metagenomic next generation sequencing data analysis while unravelling a case of uncommon campylobacteriosis
This study shows the pivotal role and strengths of mNGS in unravelling an unusual case of diarrhoea and demonstrates how mNGS can guide established microbiological methods to improve on current limitations. However, it also emphasises the need for careful interpretation of sequencing data, particularly for closely related bacterial species from clinical samples that are known to support complex microbial communities.
Authors
- Mateja Pirš (ORCID: https://orcid.org/0000-0003-2858-9876)
- Tina Triglav
- Andraž Celar Šturm
- Martin Bosilj (ORCID: https://orcid.org/0000-0002-4663-9622)
- Katja Strašek Smrdel (ORCID: https://orcid.org/0000-0003-0134-8888)
- Miša Korva (ORCID: https://orcid.org/0000-0003-1501-1004)
- Rok Kogoj (ORCID: https://orcid.org/0000-0002-8999-4923)
- Eva Kvas
- Lidija Lepen
Institutions
- University of Ljubljana (SI)
- Ljubljana University Medical Centre (SI)
- Institute of Oncology Ljubljana (SI)
Publication Details
- Journal
- Journal of Biomedical Informatics
- Published
- 2025-05-02
- DOI
- https://doi.org/10.1016/j.jbi.2025.104841
- Citations
- 1
- Primary Topic
- Salmonella and Campylobacter epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.16
Funders
- Javna Agencija za Raziskovalno Dejavnost RS