Polarization-resolved spectral focusing CARS microscopic imaging

Abstract Spectral focusing microscopy imaging method is an important spectral microscopy imaging method with wide applications in the field of coherent anti-Stokes Raman scattering (CARS) microscopy imaging research. This article used a single femtosecond oscillator to build a spectral focusing CARS (SF-CARS) microscopy imaging system, combined with polarization-resolved technology, to study the polarization-resolved SF-CARS microscopy imaging of polystyrene samples. By changing the polarization direction of the excitation light field, the vibration orientation of the sample was analyzed. By changing the polarization state of the excitation light field, effective selective excitation was achieved, and the excitation efficiency of Raman vibration of the sample was improved.