Beyond the Hydrashift assay: the utility of isoelectric focusing for therapeutic antibody and paraprotein detection
Insufficient neutralization capacity of the Hydrashift Assay was not observed within the clinically relevant range of investigated tmAb concentrations. IEF offers a simple and flexible alternative to the Hydrashift assay for distinguishing tmAbs from paraproteins. Its advantages include broader applicability to various tmAbs and independency from specific commercial platforms.
Authors
- Wolfgang Andreas Nockher
- Christoph Mann (ORCID: https://orcid.org/0000-0002-7823-8428)
- Rieke Reiter (ORCID: https://orcid.org/0000-0003-4783-9471)
Institutions
- Philipps University of Marburg (DE)
Publication Details
- Journal
- Clinical Chemistry and Laboratory Medicine (CCLM)
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1515/cclm-2025-0352
- Citations
- 1
- Primary Topic
- Multiple Myeloma Research and Treatments
- Type
- article
- Field-Weighted Citation Impact
- 1.77