Detection of respiratory syncytial virus based on RT-RPA and CRISPR-Cas12a
Human respiratory syncytial virus (hRSV) is one of the most prevalent viruses infecting children globally. In this study, we employed the RT-RPA with CRISPR/Cas12a detection methodology to detect and differentiate RSV-A and RSV-B, particularly in resource-limited settings. The detection limit for RSV-A and RSV-B was approximately 10<sup>2</sup> and 10<sup>3</sup> copies/reaction, respectively. The assay revealed 100% specificity in detecting both RSV-A and RSV-B. Diagnostic accuracy was 90.32 and 93.55% for RSV-A and RSV-B, respectively, compared to RT-qPCR. These data indicate a proficient strategy for RSV screening, demonstrating promise for prospective applications in detecting diverse viral infections.
Authors
- Oraphan Mayuramart (ORCID: https://orcid.org/0000-0001-5972-6648)
- Siripat Pasittungkul
- Pattaraporn Nimsamer (ORCID: https://orcid.org/0000-0003-3741-7558)
- Pornchai Kaewsapsak (ORCID: https://orcid.org/0000-0001-7921-0868)
- Ariya Khamwut (ORCID: https://orcid.org/0000-0002-5018-953X)
- Yong Poovorawan (ORCID: https://orcid.org/0000-0002-2337-6807)
- Sunchai Payungporn (ORCID: https://orcid.org/0000-0003-2668-110X)
- Nantinee Chomta
- Juthamas Nimnual
Institutions
- Siriraj Hospital (TH)
- Chulalongkorn University (TH)
- Mahidol University (TH)
Publication Details
- Journal
- Experimental Biology and Medicine
- Published
- 2025-05-01
- DOI
- https://doi.org/10.3389/ebm.2025.10387
- Citations
- 3
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 2.42
Funders
- Chulalongkorn University
- National Research Council of Thailand
- Thailand Science Research and Innovation
- Faculty of Medicine, Chulalongkorn University