Cellular effects of splenectomy on liver regeneration after 70% resection
Animals splenectomized prior to the liver resection showed higher rates of cell proliferation along with higher content of р53 protein in the remnant organ. Splenectomy also correlated with decreased rates of Ly6C+ monocyte and Ly6G+ leukocyte migration. Macrophages in the regenerating liver were transcriptomically enriched for signaling pathways associated with monocyte migration, cell adhesion and cell death. As shown by the GFP+ leukocyte transplantation experiment, the leukocytes migrating to the regenerating liver are mainly of splenic origin. According to high-throughput sequencing data, these cells express high levels of cell adhesion molecules. The spleen has a significant effect on liver regeneration through secretion of biologically active substances and migrating leukocytes. Pre-splenectomy leads to a more pronounced liver damage response after 70% resection, as indicated by higher rates of cell proliferation, higher p53 protein content and cell death-associated signaling pathway activation.
Authors
- Timur Fatkhudinov (ORCID: https://orcid.org/0000-0002-6498-5764)
- Andrey Elchaninov (ORCID: https://orcid.org/0000-0002-2392-4439)
- Polina Vishnyakova (ORCID: https://orcid.org/0000-0001-8650-8240)
- Evgeniya Kananykhina (ORCID: https://orcid.org/0000-0002-9779-2918)
- Evgeny Karpulevich (ORCID: https://orcid.org/0000-0002-6771-2163)
- D. Yu. Trofimov (ORCID: https://orcid.org/0000-0002-1569-8486)
- Г. Т. Сухих (ORCID: https://orcid.org/0000-0002-7712-1260)
- Maria Kuznetsova (ORCID: https://orcid.org/0000-0002-9834-9328)
- Elena Gantsova (ORCID: https://orcid.org/0000-0003-4925-8005)
- Dmitry Goldshtein (ORCID: https://orcid.org/0000-0003-2438-1605)
- Stéphanie Denis (ORCID: https://orcid.org/0000-0001-7761-8843)
- Miroslava Chirkova (ORCID: https://orcid.org/0009-0008-3659-0360)
- Victoria Karyagina
- Larkin Anatoliy
- Ibrahim Atabekov
Institutions
- National Research University Higher School of Economics (RU)
- Peoples' Friendship University of Russia (RU)
- Institute for System Programming (RU)
- Research Centre for Medical Genetics (RU)
- National Medical Research Center for Obstetrics, Gynecology and Perinatology named after Academician V.I.Kulakov of the Ministry of Healthcare of the Russian Federation (RU)
- Research Institute of Human Morphology (RU)
Publication Details
- Journal
- Frontiers in Cell and Developmental Biology
- Published
- 2025-05-01
- DOI
- https://doi.org/10.3389/fcell.2025.1561815
- Citations
- 3
- Primary Topic
- Liver Disease Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 4.06
Funders
- Russian Science Foundation