Development and validation of cost-effective multi-sample hypoxia chambers for proton ultra-high dose rate organoid irradiations
The tissue sparing of ultra-high dose rate irradiation has been partially observed <i>in vitro</i> while controlling oxygen levels. Here, we developed a hypoxia system for high-throughput irradiations that accommodates embedded 3D cultures, such as organoids and spheroids, enabling the study of the effect of oxygen on radiation responses.
Authors
- Stefan Both (ORCID: https://orcid.org/0000-0001-6652-6054)
- Johan Bussink (ORCID: https://orcid.org/0000-0002-5751-4796)
- Robert P. Coppes (ORCID: https://orcid.org/0000-0001-5503-1064)
- Ralf Köster
- Lara Barazzuol (ORCID: https://orcid.org/0000-0002-6538-2383)
- A. Gerbershagen (ORCID: https://orcid.org/0000-0002-7648-3681)
- Hans Peters (ORCID: https://orcid.org/0000-0003-4353-8970)
- Jeremy P Gunawan
- Marc‐Jan van Goethem
Institutions
- Radboud University Nijmegen (NL)
- Radboud University Medical Center (NL)
Publication Details
- Journal
- Clinical and Translational Radiation Oncology
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1016/j.ctro.2025.100970
- Citations
- 1
- Primary Topic
- Radiation Therapy and Dosimetry
- Type
- article
- Field-Weighted Citation Impact
- 1.52
Funders
- Stichting De Cock-Hadders
- Universitair Medisch Centrum Groningen
- Ministerie van Economische Zaken en Klimaat