Generation of human induced pluripotent stem cell lines from two down syndrome patients, including a down syndrome/Alzheimer’s disease case (FLENIi002-A) and a beta-amyloid-resistant case (FLENIi003-A)
Two human induced pluripotent stem cell (hiPSC) lines, FLENIi002-A and FLENIi003-A, were generated from peripheral blood mononuclear cells (PBMCs) using the lentiviral-hSTEMCCA-loxP vector. FLENIi002-A was derived from a 52-year-old Down syndrome patient with Alzheimer's disease and amyloid-beta brain accumulation. FLENIi003-A was derived from a cognitively unimpaired 51-year-old Down syndrome patient exhibiting no brain amyloid-beta deposition. Both lines retained the trisomy 21 genotype, and their pluripotency and differentiation potential were confirmed.
Authors
- Sofía Mucci
- Ezequiel Surace (ORCID: https://orcid.org/0000-0001-8492-9578)
- Leonardo Romorini (ORCID: https://orcid.org/0000-0002-3972-0991)
- Lucía Pertierra
- Nahuel Magrath
- Luciana Isaja (ORCID: https://orcid.org/0000-0003-1987-8529)
- Silvia Guzmán Vázquez (ORCID: https://orcid.org/0000-0003-0476-4289)
- Soledad Rodríguez-Varela
- Giulia S. Clas
- Belén Helou (ORCID: https://orcid.org/0000-0001-5513-010X)
- Ricardo Allegri (ORCID: https://orcid.org/0000-0001-7166-1234)
- Yanina Bérgamo (ORCID: https://orcid.org/0009-0008-5629-9853)
- Gustavo Sevlever (ORCID: https://orcid.org/0000-0002-9567-7553)
- Mariela C. Marazita (ORCID: https://orcid.org/0000-0002-6944-2486)
- María Élida Scassa (ORCID: https://orcid.org/0000-0002-2158-5306)
- Fernanda Tapajóz (ORCID: https://orcid.org/0000-0003-2432-8177)
Institutions
- Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (AR)
- Instituto de Neurología Cognitiva (AR)
Publication Details
- Journal
- Stem Cell Research
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1016/j.scr.2025.103728
- Primary Topic
- Pluripotent Stem Cells Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Fleni