Formulation and Evaluation of Biosynthesized Terminalia Arjuna Extract Loaded Polymeric Nanoparticles for the Treatment of Hyperlipidemia

The work aimed to formulate and characterize the Terminalia arjuna bark extract-loaded polymeric nanoparticles used to treat hyperlipidemia. To study the in vitro drug release from the prepared nanoparticles by static diffusion method and to carry out the in vivo anti-hyperlipidemic activity in the Wistar rat model. The Terminalia arjuna bark was dried and powdered, further through Soxhlet apparatus ethanolic extraction was carried out and fine powder was formed.Phytochemical screening and quantitative analysis wear performed.Terminalia arjuna bark extract loaded nanoparticles were prepared by polymerisation i.e., solvent evaporation method.The obtained sample of EETA (ethanolic extraction of Terminalia arjuna bark extract) was estimated for UV and IR studies.Prepared polymeric nanoparticles were characterised for FT-IR,Particle size, PDI, Zeta potential,% DEE, SEM, TEM and antioxidant activity.In vitro and In vivo drug release studies and Stability studies were also evaluated.The FT-IR study showed that drugs and polymers are compatible with each other.Particle size was found to be in the range of 188nm to 220nm.The PDI for methods was found to be 0.335 to 1.000 respectively.The % DEE of TANP4 was found to be 88%.The TEM showed that the polymeric nanoparticles prepared were found to be granular, elliptical in shape and crystalline in nature.The SEM showed irregularity of shape with smooth texture.The antioxidant activity of TANP4 showed good antioxidant property as compared to standard ascorbic acid.After 12 hours, the in vitro release research demonstrated sustained activity; a higher polymer led to a lower drug release. The produced polymeric nanoparticles exhibit notable hyperlipidemic activity, according to the in vivo investigation.According to stability studies, formulations remain stable up to three months.Terminalia arjunabark extract loaded polymeric nanoparticles was successfully prepared by solvent evaporation method.The formulated nanoparticles were characterized for various parameters and fruitfully performed in vivo activity.