Field evaluation of the Bioline Malaria Ag P.f/Pan rapid diagnostic test: causes of microscopy discordance and performance in Uganda
The Bioline Malaria Ag P.f/Pan combination RDT was found to be highly sensitive in Uganda and reliable for ruling out malaria. False negative RDT results were primarily due to low density P. falciparum infections, non-falciparum infections, or incorrect microscopy results. In contrast, false positive RDT results were common, most likely due to persistent HRP2 antigenaemia in this high transmission setting though causes of false positive RDTs were not investigated. The low specificity of HRP2-based RDTs may result in overuse of anti-malarial drugs and missed diagnoses of non-malarial febrile illnesses.
Authors
- Brian A. Kagurusi (ORCID: https://orcid.org/0000-0001-8615-6732)
- Samuel Gonahasa (ORCID: https://orcid.org/0000-0003-4865-0718)
- Victor Asua (ORCID: https://orcid.org/0000-0002-0489-4692)
- Catherine Maiteki‐Sebuguzi (ORCID: https://orcid.org/0000-0003-1471-7996)
- Melissa D. Conrad (ORCID: https://orcid.org/0000-0002-9984-8030)
- Thomas Katairo (ORCID: https://orcid.org/0000-0002-2002-8911)
- Jessica Briggs (ORCID: https://orcid.org/0000-0002-8078-3898)
- Sarah G. Staedke (ORCID: https://orcid.org/0000-0003-2505-821X)
- Jimmy Opigo (ORCID: https://orcid.org/0009-0003-1521-4159)
- Daudi Jjingo (ORCID: https://orcid.org/0000-0003-1685-9234)
- Samuel L. Nsobya (ORCID: https://orcid.org/0000-0003-4986-8823)
- Bryan Greenhouse (ORCID: https://orcid.org/0000-0003-0287-9111)
- Bosco Agaba (ORCID: https://orcid.org/0000-0003-4601-5171)
- Monica Mbabazi (ORCID: https://orcid.org/0000-0001-6621-0586)
- Moses Robert Kamya (ORCID: https://orcid.org/0000-0001-9106-4234)
- Isaac Ssewanyana (ORCID: https://orcid.org/0000-0001-8605-2001)
- Shahiid Kiyaga (ORCID: https://orcid.org/0000-0002-6380-4714)
- Stephen Tukwasibwe (ORCID: https://orcid.org/0000-0002-6469-6516)
- Grant Dorsey
- Francis D Semakuba
- Bienvenu Nsengimaana (ORCID: https://orcid.org/0009-0002-9786-7294)
- Innocent Wiringilimaana
- K.P. Hilton
- Kisakye Diana Kabbale
- Caroline Mwubaha
Institutions
- University of California, San Francisco (US)
- Liverpool School of Tropical Medicine (GB)
- University of California System (US)
- Ministry of Health (UG)
- Infectious Diseases Institute (UG)
- Infectious Diseases Research Collaboration (UG)
- Makerere University (UG)
- University of Tübingen (DE)
- Uganda Christian University (UG)
- University of California, Berkeley (US)
Publication Details
- Journal
- Malaria Journal
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1186/s12936-025-05379-6
- Citations
- 2
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 3.76
Funders
- Bill and Melinda Gates Foundation
- National Institutes of Health
- National Institute of Allergy and Infectious Diseases
- Fogarty International Center