Massilia paldalensis sp. nov., Isolated from Stream Bank Soil

A novel rod-shaped, Gram-negative, motile, aerobic and heavy metal-resistant bacterial strain, designated TN1-12T, was isolated from stream bank soil in Paldal district, Suwon City, Republic of Korea. Growth occurred at 10–40 °C (opt 30 °C), NaCl concentrations up to 2% (w/v) and pH 5.0–8.0 (opt pH 7.0). Based on the 16S rRNA gene sequence, the closest relatives of strain, TN1-12T, are Massilia putida 6NM-7T (98.21% similarity), Massilia forsythiae GN2-R2T (98.00%), Massilia rhizosphaerae NEAU-GH312T (97.79%), Massilia aurea AP13T (97.78%) and Massilia niabensis 5420S-26T (97.71%). The predominant ubiquinone is Q-8. The G+C content of the DNA is 66.7 mol%. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine. The major fatty acids are C16:0, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), 17:0 cyclo and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c). DNA–DNA hybridization and Average Nucleotide Identity data showed values below 26% and 85%, respectively, confirming that TN1-12T represents a novel species. Based on the genotypic, chemotaxonomic and physiological data presented in this study, we propose that strain TN1-12T represents a novel species within the genus Massilia with the name Massilia paldalensis sp. nov. (=KACC 23946T = CGMCC 1.65296T).