Biotin interference in routine clinical immunoassays
Our study highlights the high prevalence of elevated biotin levels in hemodialysis and ICU patients, serving as a critical reference for clinical result interpretation. We confirm that Roche's newer reagents exhibit enhanced biotin tolerance, consistent with the manufacturer's claims, and demonstrate that biotin depletion effectively restores assay accuracy. These findings provide valuable methodological guidance for mitigating biotin interference in clinical immunoassays.
Authors
- Kung-Ming Chung (ORCID: https://orcid.org/0000-0002-3084-7099)
- K.-S. Lee
- Kuo-Chun Chiu
- Chi‐Chien Kuo (ORCID: https://orcid.org/0000-0002-1892-6712)
- Ya-Fen Lee
- Wei Lü (ORCID: https://orcid.org/0000-0001-9859-8394)
- Yiming Liao (ORCID: https://orcid.org/0000-0002-7043-5953)
- Liang Cheng (ORCID: https://orcid.org/0000-0002-4173-9544)
- Ching‐Fa Yeh
- Tzu-I Chien
- Jia-Rong Jhan
- Huiyuan Yan (ORCID: https://orcid.org/0000-0003-0060-4878)
Institutions
- National Taiwan University (TW)
- National Taiwan University Hospital (TW)
Publication Details
- Journal
- Practical Laboratory Medicine
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1016/j.plabm.2025.e00472
- Citations
- 1
- Primary Topic
- Biotin and Related Studies
- Type
- article
- Field-Weighted Citation Impact
- 1.15