Identification of chlorophyll and color content in grape leaves during two growth stages (flowering and setting)

Chlorophyll concentration is a measure of the leaf's ability to photosynthesize. It is essential for advancing the physiological state of plants. A rapid estimate of in situ leaf chlorophyll concentration is frequently provided by SPAD meters. When utilizing SPAD meters to accurately quantify leaf chlorophyll content, it is important to consider the variations in growth conditions of vegetation species. This study aimed to calculate the chlorophyll (a*, b*) and L* (Lightness) levels from SPAD values in grape leaves for pickling during two vine different growth stages: blooming and set. In this study, 189 plants from Narince x Isabella combination, 39 plants from Narince x Kishmish Vatkana combination, and 218 plants from Narince x Regent combination were used. In addition, Narince, Künefi, Erciş, Dökülgen, Fenerit, Hatun Parmağı, Horoz Karası, Muhammedi, Karaerik, and Vakkas grape varieties from our local germplasm and Italia and Kyoho varieties known to be susceptible to powdery mildew were included. In this study, chlorophyll content has decreased statistically from flowering to setting for L*, a*, and b* concentration. Analyzing two factors (genotypes and periods) have statistical differences for both main and interactions. The study reported here indicates that a statistical separation ratio of chlorophyll a*/b* was discovered. According to the results of the study, concentrations of L*, a*, and b* decreased from flowering to berry set phases. This study points out that various developmental stages in plant species might affect the link between leaf chlorophyll content and SPAD readings.