Development of a multiplex loop-mediated isothermal amplification (LAMP) method for differential detection of <i>Mycobacterium bovis</i> and <i>Mycobacterium tuberculosis</i> by dipstick DNA chromatography
Human tuberculosis caused by <i>Mycobacterium tuberculosis</i> and <i>Mycobacterium bovis</i> shows similar clinical symptoms; however, the treatment differs because <i>M. bovis</i> is inherently resistant to pyrazinamide, a key first-line drug effective against <i>M. tuberculosis</i>. Most available molecular tools cannot distinguish the two biovars. This study addresses this gap by introducing a multiplex loop-mediated isothermal amplification (LAMP) method coupled with dipstick chromatography that can simultaneously and differentially detect <i>M. bovis</i> and <i>M. tuberculosis</i> within 60 min. The LAMP method does not require sophisticated high-cost equipment and can be easily implemented in resource-limited settings. Our LAMP facilitates rapid and accurate tuberculosis diagnosis, enabling appropriate therapeutic agents to be selected in areas where bovine and human tuberculosis coexist. It can also screen for <i>M. bovis</i> infection in humans and livestock, providing prevalence data in areas where such information is lacking.
Authors
- Precious Bwalya (ORCID: https://orcid.org/0000-0002-4212-4816)
- Chie Nakajima (ORCID: https://orcid.org/0000-0002-9194-1062)
- Keiko Yamada
- Thoko Flav Kapalamula (ORCID: https://orcid.org/0000-0003-1127-3138)
- Lavel C. Moonga (ORCID: https://orcid.org/0000-0001-7814-9997)
- Joseph Yamweka Chizimu (ORCID: https://orcid.org/0000-0002-2126-5759)
- Musso Munyeme (ORCID: https://orcid.org/0000-0001-7224-1071)
- Shiomi Yoshida (ORCID: https://orcid.org/0000-0002-8197-3829)
- Jeewan Thapa (ORCID: https://orcid.org/0000-0002-3447-6483)
- Stephen V. Gordon (ORCID: https://orcid.org/0000-0002-4833-5542)
- Mwangala Lonah Akapelwa (ORCID: https://orcid.org/0000-0002-8252-2798)
- Eddie Samuneti Solo
- Makoto Kawase (ORCID: https://orcid.org/0000-0001-7269-2462)
- Aki Tamaru (ORCID: https://orcid.org/0000-0002-9078-7972)
- Bernard M. Hang’ombe (ORCID: https://orcid.org/0000-0002-4201-6401)
- Yasuhiko Suzuki (ORCID: https://orcid.org/0000-0002-1313-1494)
- Takuya Kodera
- Takayuki Wada (ORCID: https://orcid.org/0000-0002-7023-7455)
- Kyoko Hayashida (ORCID: https://orcid.org/0000-0001-9266-5162)
Institutions
- University College Dublin (IE)
- Ministry of Health (ZM)
- Hokkaido University (JP)
- University Teaching Hospital (ZM)
- University of Zambia (ZM)
- Lilongwe University of Agriculture and Natural Resources (MW)
- Institut de Recherche Vaccinale (FR)
- Zambia National Public Health Institute (ZM)
- NHO Kinki Chuo Chest Medical Center (JP)
- Osaka Metropolitan University (JP)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1128/spectrum.02421-24
- Primary Topic
- Biosensors and Analytical Detection
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Japan Agency for Medical Research and Development
- Ministry of Education, Culture, Sports, Science and Technology