The role of AdhE mutations in <i>Thermoanaerobacterium saccharolyticum</i>
Many anaerobic bacteria maintain redox equilibrium by producing reduced organic compounds such as ethanol. The final two steps of ethanol production are mediated by a bifunctional enzyme, AdhE, and this enzyme is a frequent target of mutations in strains engineered for increased ethanol production. Paradoxically, these mutations increase ethanol production by eliminating the activity of one domain of the AdhE enzyme (the ADH domain). This provides additional support for a redox-imbalance theory of alcohol tolerance, which challenges the prevailing hypothesis that alcohol tolerance is associated with cell membrane effects.
Authors
- Marybeth Maloney
- Daniel G. Olson (ORCID: https://orcid.org/0000-0001-5393-6302)
- Yannick J. Bomble (ORCID: https://orcid.org/0000-0001-7624-8000)
- Lee R. Lynd (ORCID: https://orcid.org/0000-0002-5642-668X)
- Tucker Burgin (ORCID: https://orcid.org/0000-0002-3714-3537)
- João Henrique Tadini Marilhano Fabri (ORCID: https://orcid.org/0000-0002-3370-1861)
- Ángel Pech‐Canul (ORCID: https://orcid.org/0000-0002-1874-322X)
- Samantha J. Ziegler (ORCID: https://orcid.org/0000-0003-2480-513X)
- Isaiah D Richardson (ORCID: https://orcid.org/0009-0006-7728-5851)
Institutions
- Dartmouth College (US)
- Oak Ridge National Laboratory (US)
- National Laboratory of the Rockies (US)
- Universidade Estadual de Campinas (UNICAMP) (BR)
- Terra (NL)
Publication Details
- Journal
- Journal of Bacteriology
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1128/jb.00015-25
- Citations
- 2
- Primary Topic
- Algal biology and biofuel production
- Type
- article
- Field-Weighted Citation Impact
- 0.73
Funders
- Center for Bioenergy Innovation