Resistance risk asssement and molecular basis of metconazole in Fusarium pseudograminearum
The fungicide metconazole, which acts as a sterol 14α-demethylation inhibitor (DMI), can exhibit strong inhibitory effects on Fusarium pseudograminearum. However, the resistance mechanism as well as the risk that F. pseudograminearum develops resistance to metconazole is yet to be fully assessed. In this study, metconazole displayed a mean EC<sub>50</sub> value of 0.0559 μg/mL against 105 F. pseudograminearum isolates. Ten sensitive parental isolates were then subjected to fungicide adaptation to generate resistant mutants, with in vitro experiments subsequently highlighting the inferior fitness of the mutants. In addition, metconazole exhibited positive cross-resistance with both mefentrifluconazole and tebuconazole. Altogether, the results confirmed the low risk that F. pseudograminearum develops resistance to metconazole. Finally, a mutation genotype (M151T) was identified in FpCYP51B, with the mutants also overexpressing the FpCYP51 genes. Subsequent molecular docking and transformation-based experiments indicated that M151T substitution and overexpression in FpCYP51 genes conferred resistance to metconazole in F. pseudograminearum.
Authors
- Jianqiang Miao (ORCID: https://orcid.org/0000-0003-0564-5150)
- Kang Yuan
- Guixiang Li (ORCID: https://orcid.org/0000-0002-8730-0713)
- Han Jiang (ORCID: https://orcid.org/0000-0002-8233-5749)
- Yiwen Li (ORCID: https://orcid.org/0000-0003-3895-1576)
- Ling Zhang (ORCID: https://orcid.org/0000-0003-1124-3545)
- Xili Liu (ORCID: https://orcid.org/0000-0002-7859-9741)
Institutions
- North West Agriculture and Forestry University (CN)
- China Agricultural University (CN)
Publication Details
- Journal
- Stress Biology
- Published
- 2025-05-01
- DOI
- https://doi.org/10.1007/s44154-025-00221-0
- Citations
- 3
- Primary Topic
- Plant Pathogens and Fungal Diseases
- Type
- article
- Field-Weighted Citation Impact
- 3.45
Funders
- National Key Research and Development Program of China