Optimization of Preparation Process of Flavonoid Phospholipid Complex of Guava Leaves by Box-Behnken Response Surface Methodology

In this study, a flavonoid phospholipid complex of guava leaves was prepared to improve its lipid solubility and bioavailability. The flavonoid phospholipid complex of guava leaves was prepared by solvent method. Taking the recombination rate as the evaluation index, single factor investigation and Box-Behnken response surface methodology were used to screen and optimize the preparation process. The results showed that the optimal preparation process of the flavonoid phospholipid complex extract of guava leaves was as follows: total flavonoids of guava leaves: soybean phospholipids (1:2), absolute ethanol as the reaction solvent, the mass concentration of total flavonoids of guava leaves as 5 mg/mL, and the reaction was carried out at 50°C for 2 h. The recombination rate reached 72.84±0.77% (n=3). The research shows that the preparation process is simple and feasible, and the prepared total flavonoid phospholipid complex of guava leaves has good stability and improved lipophilicity.