Development of Reduced Graphene Oxide Ionic Liquid Nanocomposite for the Electrochemical Sensing of Uric Acid

Graphene-based biosensors are garnering significant scholarly interest attributable to the engineering potential of graphene, which enhances both specificity and sensitivity. However, pristine graphene doesn’t account for any specific interactions. The research investigates the utilization of reduced graphene oxide (rGO) in conjunction with hexylmethyl imidazolium tetrafluoroborate ([HMIM]/[BF4]) ionic liquid (IL) modified glassy carbon electrodes (GCE) for the electrochemical detection of uric acid. Graphene oxide (GO) was synthesized using the improved Hummer’s method. Raman spectra was recorded to confirm the exfoliation of graphite to GO and reduction of GO. Cyclic voltammetry and electrochemical impedance spectroscopy were used to study the electron transfer kinetics of the modified electrode. The linearity range of the fabricated sensor was recorded using differential pulse voltammetry (DPV) and observed linearity from 19.6 × 10–6 M to 1.6 × 10–2 M of uric acid concentration with a detection limit of 5.1 × 10–6 M using differential pulse voltammetry (DPV).