Physicochemical Characteristics of Chitosan Extracted from Pleurotus ostreatus and Its Anticancer Activity Against the MDA-MB-231 Breast Cancer Cell Line

One of the main causes of death worldwide is cancer, a disorder in which a solid or liquid mass of cells known as a tumor develops when one or more cells lose the capacity to regulate their development. This study aims to assess the potential of chitosan as an anticancer agent in place of standard therapy regimens that have some degree of unselective cytotoxicity. PCR was performed for the RNA extraction of Caspase-3 and β-actin genes, and Cq values and quantification curves for each gene were recorded. Additionally, SRB and FITC apoptosis investigations were used to assess the effectiveness of chitosan powder's anticancer activity against breast cancer cells.