Evaluation of the APTIMA Combo 2™ kit for the detection of Chlamydia trachomatis and Neisseria gonorrhoeae in frozen semen
Detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) in cryopreserved semen is crucial for screening sperm donors. The evaluation of the limit of detection (LOD) of the APTIMA Combo 2™ kit (Panther, Hologic) was performed on cryopreserved semen samples spiked with CT and NG at concentrations ranging from 1 to 106 IFU/mL or CFU/mL, respectively. The LOD was 102 IFU/mL for CT and 10 CFU/mL for NG in single infection or coinfection. An inhibitory effect of semen on amplification was highlighted. This study confirmed the performance of the APTIMA Combo 2™ kit for screening cryopreserved sperm samples before donation.
Authors
- Robin Chautard
- Sabine Pereyre (ORCID: https://orcid.org/0000-0003-1757-4206)
- Sébastien Hantz (ORCID: https://orcid.org/0000-0003-2635-0773)
- Maxime Lafontaine
- Cécile Bébéar (ORCID: https://orcid.org/0000-0001-6065-2332)
- Fabien Garnier (ORCID: https://orcid.org/0000-0002-7749-952X)
- Olivia Peuchant (ORCID: https://orcid.org/0000-0002-5358-8990)
- Arabella Touati (ORCID: https://orcid.org/0000-0002-9369-4931)
- Merve Deniz Genç (ORCID: https://orcid.org/0009-0007-9729-3258)
- T Lutz
Institutions
- Centre Hospitalier Universitaire de Bordeaux (FR)
- Centre Hospitalier Universitaire de Limoges (FR)
- Université de Limoges (FR)
Publication Details
- Journal
- European Journal of Clinical Microbiology & Infectious Diseases
- Published
- 2025-04-30
- DOI
- https://doi.org/10.1007/s10096-025-05138-0
- Citations
- 2
- Primary Topic
- Sperm and Testicular Function
- Type
- article
- Field-Weighted Citation Impact
- 3.40
Funders
- Université de Limoges