SAT-457 In-Vitro Effects of Bisphenol A, Benzophenones 2 and 3 on Pro-inflammatory Protein Expression in Hemi-Hypothalami From Adult Male Mice

Abstract J.M. Riaño Gómez: None. T.J. Alcaraz Clouet: None. V.C. De Miguel: None. V.A. Lux-Lantos: None. E.M. Sorianello: None. M.O. Fernandez: None. According to a recent report from the United Nations Environment Programme and the Basel, Rotterdam and Stockholm conventions secretariat (1), it has been estimated that around 13,000 chemicals are involved in the production of plastics. Among these chemicals, we find monomers, additives and non-intentionally added substances. Several chemicals used in plastics are cataloged as endocrine disrupting chemicals or EDCs. Examples of EDCs are Bisphenol A (BPA), a monomer of polycarbonate plastics, and Benzophenones (BPs), UV-filters. Our hypothesis is that exposure to these EDCs causes brain inflammation and predisposes to obesity and infertility. Previous results showed that the in-vitro exposure to BPA increased mRNA expression levels of Glial Fibrillary Acidic Protein (GFAP) in whole hypothalami from adult male mice, and IL-18 in mature GnRH neurons, GT1-7 cells. Here, protein expression of pro-inflammatory markers (Iba1 and GFAP) was evaluated in hemi-hypothalami from adult C57 male mice. Hypothalami were incubated in Krebs-Ringer medium in the presence or absence of BPA, BP2 or BP3 (1x10-9 M) or medium alone (C) for eight hours. Tissues were lysed in RIPA buffer with protease inhibitors and protein concentration measured by Bradford. Proteins were separated in polyacrylamide gels and transferred to PVDF membranes. Iba1, GFAP and tubulin were detected using specific antibodies. Results were expressed as Media±SEM and analyzed by Paired t-test (Statistica). Exposure to all EDCs increased protein expression of Iba1 (Iba1, C=1.0±0.1, BPA=1.5±0.1, Paired t-test p<0.05, n=7; C=0.9±0.1, BP2=1.1±0.1, Paired t-test p<0.05, n=7; C=0.9±0.1, BP3=1.3±0.2, Paired t-test p<0.05, n=7). Exposure to BP2 increased GFAP compared to C (C=1.0±0.1; BP2=1.3±0.1, Paired t-test p<0.05, n=7). Neither BPA nor BP3 changed GFAP protein expression. These results will help us to understand how exposure to EDCs affects brain inflammation. More studies are underway to further explore the observed effects. Supported by: CONICET, ANPCyT, Int. Soc. for Neurochemistry, Fund. René Barón, Fund. Williams, Fund. H. Bigand. Reference; 1. BRS. Global governance of plastics and associated chemicals. Secretariat of the Basel, Rotterdam and Stockholm Conventions, United Nations Environment Programme, Geneva. Raubenheimer K, Urho, N. 2023. Saturday, June 1, 2024