Neural Activity During Call Production in the Female Zebra Finch Homolog of the Male Forebrain Song System

Female zebra finches (Taeniopygia guttata) are unable to sing because of the vestigial development of forebrain song control areas such as the RA (nucleus robustus archistriatalis), a premotor nucleus of the song control pathway. In male zebra finches, RA is also involved in call-based vocal communication in addition to song control. Here, we monitored the activity of RA neurons during vocal communication in freely behaving females using a miniaturized telemetric recording device combined with telemetric audio recording. Neurons in the RA region showed premotor activity associated with stack and tet calls, two innate short-range social calls produced by both sexes. RA units were active when females called to respond to a male partner's call or to initiate a partner's call. However, spontaneous, regularly firing units, typical of male RA, were very rare in females or, when found, showed no association with vocal output. Despite the small number of adult female RA neurons, these neurons are not functionless, but are involved in call-based communication.