Carotenoid pigments of Kocuria flava PUTS1_3 isolated from sediments of Puttalam lagoon mangrove ecosystem, Sri Lanka exhibit bioactive properties

Microorganisms, inhabiting various ecological niches, exhibit a capacity to produce a diverse array of pigments with different shades. These colorful microbial pigments may also potentially possess beneficial bioactivities. This dual functionality together with the ease of mass production and downstream processing has shifted the global attention towards the use of microbially-derived pigments as bioactive colorants in different industries. Therefore, the present study was conducted with the aim of characterizing the pigments from Kocuria flava and identifying their potential biotechnological applications. The bacterium, PUTS1_3, was isolated using the surface sediment samples from the Puttalam mangrove ecosystem, Sri Lanka and it was identified as Kocuria flava using 16S rRNA gene sequencing. The yellow, intracellular pigment of PUTS1_3 was obtained by treating the cell pellet with methanol. Characterization of the pigment extract using UV-visible spectroscopy, TLC, and HPLC confirmed the presence of three carotenoid compounds, including β-carotene. The pigment extract also demonstrated antibacterial activity, against Gram positive bacteria tested. Antioxidant properties were observed with an IC₅₀ value of 181.95 ± 4.57 µg/ml in the DPPH free radical scavenging assay. Although its sun protection factor was comparatively low (SPF 7.69 ± 0.01), the pigment showed promising results as a textile dye demonstrating good color performance and stability in washing and pH stability tests. Moreover, fabrics dyed with the pigment extract displayed antibacterial activity against Staphylococcus aureus (ATCC 25923). These findings suggest the potential use of the yellow pigments of K. flava PUTS1_3 for various biotechnological applications.