Generation of transgene-free genome-edited carrot plants using CRISPR/Cas9-RNP complexes
Key MessageWe report a process by which transgene-free, gene-edited carrot plants can be efficiently produced by introducing Cas9 ribonucleoprotein complexes into protoplasts and regenerating carrot plants from those protoplasts.
Authors
- Rajesh Yarra (ORCID: https://orcid.org/0000-0002-7208-5168)
- Patrick J. Krysan (ORCID: https://orcid.org/0000-0003-4916-915X)
Institutions
- University of Wisconsin–Madison (US)
Publication Details
- Journal
- Plant Cell Reports
- Published
- 2025-04-29
- DOI
- https://doi.org/10.1007/s00299-025-03499-6
- Citations
- 3
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 2.42
Funders
- U.S. Department of Agriculture
- National Institute of Food and Agriculture