Characterizing spatial immune architecture in metastatic melanoma using high-dimensional multiplex imaging
Our results reveal stage-specific shifts in immune composition and spatial organization within the melanoma TIME. In advanced disease, immunosuppressive neighborhoods emerge alongside changes in immune cell localization, with spatial patterns of immune coordination-particularly involving CTLs, NK cells, and B cells-strongly predicting survival. These findings highlight spatial biomarkers that may refine patient stratification and guide combination immunotherapy strategies targeting the inflammatory architecture of the TIME.
Authors
- Regan Baird
- Jared K. Burks (ORCID: https://orcid.org/0000-0002-6173-9074)
- Joel Eliason (ORCID: https://orcid.org/0000-0003-2227-8727)
- Dave S.�B. Hoon (ORCID: https://orcid.org/0000-0003-1915-3683)
- Sühendan Ekmekçioğlu
- Yasunari Fukuda (ORCID: https://orcid.org/0000-0002-5550-7736)
- Santhoshi Krishnan (ORCID: https://orcid.org/0000-0001-5114-9778)
- Dan Winkowski
- Michael A. Davies (ORCID: https://orcid.org/0000-0002-0977-0912)
- Sung-Nam Cho (ORCID: https://orcid.org/0000-0002-1115-8247)
- Elizabeth A. Grimm (ORCID: https://orcid.org/0000-0001-7140-0587)
- Matías A. Bustos (ORCID: https://orcid.org/0000-0001-9625-1127)
- Akshay Basi
- Arvind Rao (ORCID: https://orcid.org/0000-0001-6115-2321)
Institutions
- The University of Texas MD Anderson Cancer Center (US)
- Nara University (JP)
- University of Michigan (US)
- Nara City Hospital (JP)
- Nara Medical University Hospital (JP)
- DHI (DK)
- Kindai University (JP)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-04-29
- DOI
- https://doi.org/10.3389/fimmu.2025.1560778
- Citations
- 4
- Primary Topic
- Immunotherapy and Immune Responses
- Type
- article
- Field-Weighted Citation Impact
- 3.19