Structure and stability of phycocyanin from thermotolerant <i>Oscillatoria</i>
Phycocyanin (PC), a pigment-protein complex with diverse biotechnological applications, plays a key role in light energy transfer for photosynthesis in cyanobacteria. PC (O-PC) from a thermotolerant cyanobacteria Oscillatoria sp. N09DM exhibits remarkable stability compared to its mesophilic counterparts, making it highly valuable for industrial and medical applications. To understand the basis of its stability, the crystal structure of O-PC is solved and analysed. Structural analysis reveals a key molecular interaction, including hydrogen bonds, salt bridges and hydrophobic interactions, along with amino acid substitutions that provide the thermal stability. Additionally, structural results provide insights into chromophore-protein interactions for understanding O-PC's role in the efficient transfer of light energy.
Authors
- Vinay Kumar (ORCID: https://orcid.org/0000-0002-7619-6236)
- Niraj Singh (ORCID: https://orcid.org/0000-0002-2365-2314)
- Ravi R. Sonani (ORCID: https://orcid.org/0000-0002-6212-2869)
- Seema Amin (ORCID: https://orcid.org/0000-0002-1112-3159)
- Stuti Patel (ORCID: https://orcid.org/0000-0002-2445-2680)
- Gagan D. Gupta (ORCID: https://orcid.org/0000-0003-2549-3190)
- Datta Madamwar (ORCID: https://orcid.org/0000-0003-3301-1120)
- Chandni Upadhyaya (ORCID: https://orcid.org/0000-0001-8601-6203)
- Bhargavi Sonavane (ORCID: https://orcid.org/0000-0002-5589-9039)
Institutions
- Bhabha Atomic Research Centre (IN)
- Government of Gujarat (IN)
- Charotar University of Science and Technology (IN)
- University of Virginia (US)
- Sardar Patel University (IN)
Publication Details
- Journal
- FEBS Letters
- Published
- 2025-04-29
- DOI
- https://doi.org/10.1002/1873-3468.70048
- Citations
- 1
- Primary Topic
- Photosynthetic Processes and Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- Gujarat Council on Science and Technology