Creation of tomato breeding material with resistance genes based on lines with functional male sterility

Relevance. Growing tomatoes in the Krasnodar Territory in the open ground is more expensive compared to other regions. One of the main reasons is the expensive system of protection against diseases that are widespread on tomatoes. The most dangerous diseases are late blight, alternariasis and fusariasis, during epiphytotic years, fruit yield losses can reach up to 90%. These diseases are caused by pathogenic fungi and oomycetes. The most effective way to combat them is to create highly tolerant hybrids. This is especially true for the south of Russia, where weather conditions are favorable for the development of diseases and the stress load on plants is maximum The purpose of the study. To create a tomato breeding material (Solánum lycopérsicum) of various uses based on lines with functional male sterility with genes of resistance to the most harmful diseases. Materials and methods . Since 2018, the Department of Horticulture has begun work on introducing various target names of genetic material from donors with pathogen resistance genes: late blight, fusarium and alternariasis into the line with a statement on functional male sterility. The methods of classical breeding were used in the breeding work. At the first stage, the Ph-3, I-2 and Asc genes were identified, providing resistance to these diseases in the breeding material available in the department of horticulture of the Federal State Budgetary Institution "FNC Ris". In 2023, 188 inbred lines were analyzed for the presence and allelic location of resistance genes to the Murray and Thompson method. Results. According to the results of the study, breeding material with genes of resistance to pathogens has been identified, the work on the evaluation of which will continue. Among the sterile lines, 4 samples (101- 2, 104-2, 106-1 and 107-2) have genes for resistance to late blight (Ph3), 2 samples (100-2 and 124-6) – to fusarium wilt (I-2) and 4 samples (91-1, 101-2, 105-1, 106-1) to alternariasis (Asc), among the lines-pollinators have genes of resistance to late blight in samples: 127-3, 129-4, 137-5, 140-1; to fusarium: 127-3, 99-5, 137-5; to alternariasis: 94-5, 92-3 and 95-2. 4 samples have a complex of resistance genes – FMS lines: 101- 2, 106-1 (Asc, Ph3); pollinator lines: 127-3 and 137-5 (I-2, Ph3). The highlighted lines differ in shape, weight and color of the fruits.