On-the-fly adaptive SNR protocol to accelerate Brillouin microscopy
This article presents an innovative method for accelerating Brillouin microscopy imaging. The proposed technique, called One-pass, dynamically adjusts the signal-to-noise ratio (SNR) during data acquisition. It identifies essential spectra in real-time and adapts the laser exposure time accordingly. This approach considerably reduces acquisition time (by a factor around 2.2 and 3.5) while maintaining acceptable image quality, as demonstrated on simulations and real biological samples (HeLa and SK-N-SH cells). The method improves the stability and efficiency of Brillouin imaging compared with conventional techniques, i.e., raster scan with a single long exposure time or the recently introduced two-pass approach.
Authors
- Emanuele Pontecorvo (ORCID: https://orcid.org/0000-0002-0084-6677)
- Matthieu Loumaigne (ORCID: https://orcid.org/0000-0002-7396-9352)
- Fabrizio Gala (ORCID: https://orcid.org/0000-0002-2172-5815)
- Giancarlo Ruocco
- Cyril Ruckebusch (ORCID: https://orcid.org/0000-0001-8120-4133)
- Nizar Bouhlel (ORCID: https://orcid.org/0000-0002-4861-8550)
- Valentin Gilet
- Li Zhang (ORCID: https://orcid.org/0000-0001-9917-4518)
- David Rousseau (ORCID: https://orcid.org/0000-0002-7935-1609)
- Léo Brechet
Institutions
- Université Fédérale de Toulouse Midi-Pyrénées (FR)
- Université de Lille (FR)
- Institut Agro Rennes-Angers (FR)
- Italian Institute of Technology (IT)
Publication Details
- Journal
- Optics Express
- Published
- 2025-04-29
- DOI
- https://doi.org/10.1364/oe.560295
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Commission
- Agence Nationale de la Recherche
- HORIZON EUROPE Framework Programme
- HORIZON EUROPE European Innovation Council