Enrichment of human IgA-coated bacterial vesicles in ulcerative colitis as a driver of inflammation
The gut microbiome contributes to chronic inflammatory responses in ulcerative colitis (UC), but molecular mechanisms and disease-relevant effectors remain unclear. Here we analyze the pro-inflammatory properties of colonic fluid obtained during colonoscopy from UC and control patients. In patients with UC, we find that the pelletable effector fraction is composed mostly of bacterial extracellular vesicles (BEVs) that exhibit high IgA-levels and incite strong pro-inflammatory responses in IgA receptor-positive (CD89<sup>+</sup>) immune cells. Biopsy analyses reveal higher infiltration of CD89<sup>+</sup> immune cells in the colonic mucosa from patients with UC than control individuals. Further studies show that IgA-coated BEVs, but not host-derived vesicles nor soluble IgA, are potent activators of pro-inflammatory responses in CD89<sup>+</sup> cells. IgA-coated BEVs also exacerbate intestinal inflammation in a dextran sodium sulfate colitis model using transgenic mice expressing human CD89. Our data thus implicate a link between IgA-coated BEVs and intestinal inflammation via CD89<sup>+</sup> immune cells, and also hint a potential new therapeutic target for UC.
Authors
- Georg Busslinger (ORCID: https://orcid.org/0000-0002-7851-9895)
- Herbert Strobl (ORCID: https://orcid.org/0000-0002-8070-4977)
- Carmen Tam‐Amersdorfer (ORCID: https://orcid.org/0000-0003-3123-6342)
- Leo Eberl (ORCID: https://orcid.org/0000-0002-7241-0864)
- Gregor Gorkiewicz (ORCID: https://orcid.org/0000-0003-1149-4782)
- Christina Passegger
- Lukas Binder (ORCID: https://orcid.org/0000-0003-4309-9808)
- Andreas Blesl (ORCID: https://orcid.org/0000-0002-9338-4140)
- Dominik Fleischhacker (ORCID: https://orcid.org/0009-0009-0799-0709)
- Kristina Schild-Prüfert (ORCID: https://orcid.org/0000-0002-0154-7310)
- Stefan Schild (ORCID: https://orcid.org/0000-0001-7842-0177)
- Ellen L. Zechner (ORCID: https://orcid.org/0000-0003-2035-1898)
- Ratchara Kalawong (ORCID: https://orcid.org/0000-0001-9389-4899)
- Christoph Högenauer (ORCID: https://orcid.org/0000-0003-4566-0806)
- Himadri B Thapa (ORCID: https://orcid.org/0000-0001-8965-6716)
- Yi‐Chi Chen (ORCID: https://orcid.org/0000-0003-3819-4085)
- Paul Kohl
- Michael R Gerstorfer
- Jana Strahlhofer
Institutions
- Austrian Academy of Sciences (AT)
- University of Graz (AT)
- Medical University of Graz (AT)
- University of Zurich (CH)
- Graz University of Technology (AT)
- BioTechMed-Graz (AT)
- CeMM Research Center for Molecular Medicine (AT)
- Austrian Agency for Health and Food Safety (AT)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-04-29
- DOI
- https://doi.org/10.1038/s41467-025-59354-5
- Citations
- 12
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 9.67
Funders
- Austrian Science Fund
- Karl-Franzens-Universität Graz
- Medizinische Universität Graz
- BioTechMed-Graz
- CSL Limited