Dynamic Thiol / Disulfide Homeostasis In Patients With Nasal Polyps And The Effects Of Smoking On Homeostasis Parameters

Abstract Objective: The study aimed to determine the dynamic thiol/disulfide homeostasis for measuring oxidative stress and the impacts of smoking on these parameters in nasal polyps (NP) patients. Materials and methods: A total of forty NP patients and 36 healthy volunteers participated in the current study. Participants were categorized into two groups: 20 smokers and 20 non-smokers. Erel and Neşelioğlu developed an automated method to analyze thiol-disulfide homeostasis parameters in samples of serum from the participants. Groups were compared. Each parameter related to thiol/disulfide homeostasis—native thiol (SH), total thiol (ToSH), disulfide (SS), SS/SH (%), SH/ToSH (%), and SS/ToSH (%)—was evaluated separately. Results: There were notable differences across the groups relating markers associated with thiol-disulfide balance. Total Thiol (ToSH) µmol/L (p=0.005), Native Thiol (SH) µmol/L (p=0.001), and SH/ToSH (%) levels were lower in patients with nasal polyps than the control group, and disulfide (SS) (p=0.001), SS/NT (%) (p=0.001), and SS/ToSH (%) levels were statistically significantly higher than the control group (p=0.001). Conclusion: In NP patients, thiol/disulfide homeostasis shifts towards disulfide formation because of native thiol oxidation. Also, parameters of Thiol/disulfide homeostasis can serve as new oxidative stress markers in nasal polyps.