Isolation and Characterization of E8 Monoclonal Antibodies from Donors Vaccinated with Recombinant Vaccinia Vaccine with Efficient Neutralization of Authentic Monkeypox Virus
E8-targeting mAbs exhibited broad-spectrum neutralization against orthopoxviruses, demonstrating therapeutic potential against both historical (VACV) and emerging (MPXV) pathogens. However, MPXV's resistance to complement-dependent enhancement highlights the necessity for pathogen-adapted optimization. These findings establish E8 as a critical conserved target for pan-poxvirus VACV and MPXV countermeasure development.
Authors
- Xiuli Shen (ORCID: https://orcid.org/0000-0002-3434-3373)
- Ying Liu (ORCID: https://orcid.org/0000-0002-9391-9707)
- Junjie Zhang (ORCID: https://orcid.org/0009-0003-7814-7889)
- Dan Li (ORCID: https://orcid.org/0000-0003-1596-8033)
- Dong Wang (ORCID: https://orcid.org/0000-0001-5137-0771)
- Shuo Wang (ORCID: https://orcid.org/0000-0002-7990-7515)
- Ran Chen (ORCID: https://orcid.org/0000-0002-8967-7258)
- Jun Zhang (ORCID: https://orcid.org/0000-0002-9650-112X)
- Shuhui Wang (ORCID: https://orcid.org/0000-0002-5931-0527)
- Yuyu Fu
- Yanling Hao (ORCID: https://orcid.org/0000-0002-2562-6849)
- Yiming Shao (ORCID: https://orcid.org/0000-0002-9416-190X)
- Yutao Shi
Publication Details
- Journal
- Vaccines
- Published
- 2025-04-27
- DOI
- https://doi.org/10.3390/vaccines13050471
- Citations
- 2
- Primary Topic
- Poxvirus research and outbreaks
- Type
- article
- Field-Weighted Citation Impact
- 2.07
Funders
- National Natural Science Foundation of China
- Chinese Center for Disease Control and Prevention
- Centers for Disease Control and Prevention