Enhancing TB diagnosis: Improving specificity with scFv antibodies targeting the PPE17 epitope
A fast and accurate indirect immunofluorescence assay was developed to identify Mtb bacteria in TB sputum smears using specific anti-PPE17 scFvs. The results illustrated the capability of both scFvs in detecting Mtb in TB samples and differentiating Mtb from Nocardia smears. This suggests the potential for a novel diagnostic test that ensures precise TB detection in sputum samples, thereby preventing any potential misdiagnosis of tuberculosis.
Authors
- Amirhossein Sahebkar (ORCID: https://orcid.org/0000-0002-8656-1444)
- Kamran Heidarnejad (ORCID: https://orcid.org/0000-0002-5962-9052)
- Foroogh Nejatollahi (ORCID: https://orcid.org/0000-0002-8928-8519)
- Seyed Nooreddin Faraji (ORCID: https://orcid.org/0000-0002-1726-8316)
- Setareh Moazen
- Mehrzad Bahtouee (ORCID: https://orcid.org/0000-0002-2750-7819)
- Farhad Abbasi (ORCID: https://orcid.org/0000-0001-9700-4849)
Institutions
- University of British Columbia (CA)
- Shiraz University of Medical Sciences (IR)
- Bushehr University of Medical Sciences (IR)
- Chitkara University (IN)
Publication Details
- Journal
- Tissue and Cell
- Published
- 2025-04-29
- DOI
- https://doi.org/10.1016/j.tice.2025.102933
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Shiraz University of Medical Sciences