Bronchial smooth muscle extracellular vesicles interfere with bronchial epithelium metabolism and function in asthma
Bronchial smooth muscle (BSM) remodeling is an important feature of severe asthma pathophysiology. We previously showed that asthmatic BSM is metabolically different and increased rhinovirus (RV) replication rate, the main trigger of severe asthma exacerbations. Extracellular vesicles (EVs) are the key mediator in cell-cell communication, but the role of BSM cells-derived EVs on bronchial epithelial has never been investigated in asthma. Using severe asthmatic and non-asthmatic tissue collection, we show that asthmatic BSM cells are able to produce a greater amount of EVs containing metabolites involved in bioenergetics. We study the bronchial epithelium energetic rewiring following stimulation with asthmatic BSM cells-derived EVs. Modifications of bronchial epithelium metabolic behavior were associated with an increased ATP production and a breakdown of bronchial epithelium barrier function such as ciliary beating frequency and efficiency. Finally, we show that asthmatic BSM cells-derived EVs increased RV replication in bronchial epithelium following RV infection.
Authors
- Edmée Eyraud (ORCID: https://orcid.org/0000-0003-3807-8359)
- Olga Ousova
- Hugues Bégueret
- Fabien Beaufils (ORCID: https://orcid.org/0000-0001-5224-7505)
- Rémi Dubois (ORCID: https://orcid.org/0000-0002-3320-6086)
- Nina Lager-Lachaud
- Pauline Estèves (ORCID: https://orcid.org/0000-0002-4787-339X)
- Jean‐William Dupuy (ORCID: https://orcid.org/0000-0002-2448-4797)
- Thierry Lesté-Lasserre (ORCID: https://orcid.org/0000-0001-8328-3816)
- Guillaume Cardouat (ORCID: https://orcid.org/0000-0002-2990-8907)
- Matthieu Thumerel (ORCID: https://orcid.org/0000-0003-4673-8433)
- Roger Marthan (ORCID: https://orcid.org/0000-0001-5412-5712)
- Floriant Bellvert (ORCID: https://orcid.org/0000-0002-3259-8655)
- Sabrina Lacomme (ORCID: https://orcid.org/0000-0003-0752-1362)
- Pierre‐Olivier Girodet (ORCID: https://orcid.org/0000-0002-9298-9711)
- Patrick Berger (ORCID: https://orcid.org/0000-0003-4702-0343)
- Thomas Trian (ORCID: https://orcid.org/0000-0002-2567-4867)
- Marilyne Campagnac
- Clément Bouchet
- Elisa Celle
- Amine Chahin
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université de Bordeaux (FR)
- Inserm (FR)
- Université Fédérale de Toulouse Midi-Pyrénées (FR)
- Institut National des Sciences Appliquées de Toulouse (FR)
- Centre Hospitalier Universitaire de Bordeaux (FR)
- Toulouse Mathematics Institute (FR)
- Neurocentre Magendie (FR)
- Centre de Recherche Cardio-Thoracique de Bordeaux (FR)
Publication Details
- Journal
- iScience
- Published
- 2025-04-29
- DOI
- https://doi.org/10.1016/j.isci.2025.112546
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Agence Nationale de la Recherche
- AstraZeneca France