Extraction-Optimization of Hydrogel from Lallemantia Royleana Seeds Through Response Surface Methodology

The aim of this study was to evaluate the parameters for the maximum extraction of hydrogel (LRH) from the Lallemantia royleana seeds by Box Behnken Design Response Surface Methodology (BBD-RSM). The influence of different parameters such as pH, temperature, water-to-seed ratio (S/W ratio) and water-to-seed contact-time (S/W time) on LRH yield was evaluated. ANOVA p-values showed that these factors were the most significant constraints of the LRH yield. The Scenarios where the Temperature, Concentration and Time are involved in optimizing the aforementioned parameters were generated in the Construct 2 Design-Expert software, which is also capable of producing 2D and 3D contour maps. The maximum LRH yield of 13.73%, was observed when the seed-to-water ratio, contact period and pH were varied in a controlled manner using the RSM-BBD method. The optimal extraction conditions for the production of LRH were a temp. of extraction of 80 degree celsius, pH of 7, contact time for S/W ratio of five hours and S-W ratio of 1:20. The second order polynomial equations were also discovered to fit better the response under consideration.