Development and performance evaluation of a multiplex fluorescent PCR method for the detection of fastidious pathogens causing respiratory infections in children
Compared to conventional bacterial culture methods, the multiplex fluorescent PCR method with SYBR Green as the DNA dye is a sensitive and cost-effective approach for the simultaneous and rapid identification of five fastidious bacteria, making it a valuable alternative for clinical diagnostic laboratories.
Authors
- S. Zhang
- Yijun Zhu (ORCID: https://orcid.org/0000-0001-9585-1235)
- Xiaoyun Shan
- Jingchao Shi (ORCID: https://orcid.org/0009-0005-8591-9706)
- Weixian Yang
Institutions
- Jingzhou Maternal and Child Health Hospital (CN)
- Guang Fu Hospital (CN)
- Jinhua Central Hospital (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- The Journal of Infection in Developing Countries
- Published
- 2025-04-29
- DOI
- https://doi.org/10.3855/jidc.20282
- Citations
- 1
- Primary Topic
- Mycobacterium research and diagnosis
- Type
- article
- Field-Weighted Citation Impact
- 1.35
Funders
- Jinhua Science and Technology Bureau
- Natural Science Foundation of Zhejiang Province