Comparative evaluation of methods for isolating extracellular vesicles from ICC cell culture supernatants: Insights into proteomic and glycomic analysis
Quantitative proteomic and glycomic analysis of ICC-derived EVs revealed that ST6GAL1 overexpression led to significant alterations in proteins involved in cancer cell adhesion and glycosylation pathways, along with specific changes in N-glycan structures. Notably, these modifications extended beyond α2,6-sialylation, suggesting that interactions between glycosyltransferases and glycans may drive these alterations.
Authors
- Chenjun Huang (ORCID: https://orcid.org/0000-0003-3836-6262)
- Linlin Wu (ORCID: https://orcid.org/0009-0008-0551-949X)
- Chunfang Gao (ORCID: https://orcid.org/0000-0002-4891-2944)
- Long Tian (ORCID: https://orcid.org/0000-0002-6318-7517)
- Mengmeng Wang (ORCID: https://orcid.org/0000-0002-9890-8900)
- Xiao Xiao (ORCID: https://orcid.org/0000-0002-4328-0634)
- Bin Fu (ORCID: https://orcid.org/0000-0001-8567-2997)
- Xuewen Xu (ORCID: https://orcid.org/0000-0003-0567-2749)
- Yueping Zhan (ORCID: https://orcid.org/0000-0002-1282-0052)
- Yueyue Li
- Jiao Wei (ORCID: https://orcid.org/0009-0007-6662-6830)
Institutions
- Fudan University Shanghai Cancer Center (CN)
- Shanghai University of Traditional Chinese Medicine (CN)
- Yueyang Hospital (CN)
Publication Details
- Journal
- Cell Communication and Signaling
- Published
- 2025-04-29
- DOI
- https://doi.org/10.1186/s12964-025-02207-x
- Citations
- 3
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 2.42
Funders
- National Science Foundation
- National Natural Science Foundation of China
- Fudan University
- Shanghai Medical College, Fudan University