Inheritance and Phenotypic Stability of Homoeolog-Edited Mutants in Hexaploid <i>Solanum nigrum</i>

These developments offer significant potential for manipulating desirable traits in polyploid crops.Particularly, CRISPR/Cas and other gene-editing technologies allow for simultaneously targeting multiple genes, introducing genetic variation that can drive new trait expression (Zhang et al. 2021).However, polyploid inheritance is more complex than that of diploids.In polyploid genomes, homoeologous genes, which are derived from different ancestral species, interact dynamically to maintain functional balance.This phenomenon, often referred to as subgenome dominance, underscores the complexity of editing polyploid genomes (Bird et al. 2018).Targeted homoeolog editing not only introduces novel allele combinations but also affects dose sensitivity, a critical factor in phenotypic expression.Dose sensitivity arises from the varying contribution of multiple homoeologs to specific traits, where the loss or modification of one homoeolog can be compensated or exacerbated by others (Birchler et al. 2012).This interaction can result in non-linear phenotypic effects, particularly -