Method development and clinical validation of LAMP-CRISPR/Cas12a for rapid detection of respiratory pathogens in children

The integrated LAMP-CRISPR/Cas12a approach represents a significant advancement in detecting respiratory pathogens in clinical settings. It offers a rapid, sensitive, and specific diagnostic tool for identifying SP and MP, which is crucial for guiding precision therapies and improving patient outcomes. Future research aims to optimize assay sensitivity, streamline workflow to minimize contamination risks, and expand its detection scope so that other types of pathogens and mutation resistance genes can be detected. This molecular diagnostic strategy holds promise for the management of respiratory infections by enabling early and precise pathogen identification.