Effect of Andrographis paniculata and Melia azadirachta against the Dengue virus

Dengue infection is emerging as a global threat due to increased mortality and morbidity, especially in tropical and sub-tropical regions. Therefore, it is of interest to evaluate the antiviral activity of <i>Andrographis paniculata</i> and <i>Melia azadirachta</i> against <i>Dengue virus</i>-1 in a vero cell line using a cytopathic effects (CPE) reduction assay and cytotoxicity assessed by MTT assay. GC-MS analysis was conducted to identify phytochemicals in the plant extracts. Both extracts showed potent antiviral activity at 150 µg/ml and were non-toxic up to 1.5 mg/ml, with 100% cell viability. These findings suggest that <i>A. paniculata</i> and <i>M. azadirachta</i> are promising candidates against <i>Dengue virus</i>-1, warranting further investigation into their active compounds.